Cells were in that case fixed for one hour with 70% methanol/acetic acidity and stained for a quarter-hour with Crystal violet in methanol. p53 and following p53-mediated results in liposarcoma. Components and Strategies Clonogenic assays, immunoblotting, stream cytometry/fluorescence-activated cell sorting, and senescence assays had been used in liposarcoma cell lines after co-treatment with nutlin-3 and … Continue reading Cells were in that case fixed for one hour with 70% methanol/acetic acidity and stained for a quarter-hour with Crystal violet in methanol